MOSA - Medical Oxygen Society of the Americas

1Address for correspondence: Department of Cellular Stress Biology, Roswell Park Cancer Institute, Carlton and Elm Sts., Buffalo, NY 14263; harold.box@roswellpark.org

Singlet Oxygen-Induced DNA Damage

Study to Determine if 102 Singlet Oxygen Induces DNA Damage

Abstract

DeFedericis, H-C., Patrzyc, H. B., Rajecki, M. J., Budzinski, E. E., Iijima, H., Dawidzik, J. B., Evans, M. S., Greene, K. F. and Box, H. C. Singlet Oxygen-Induced DNA Damage. Radiat. Res. 165, 445–451 (2006).

Singlet oxygen, hydrogen peroxide, hydroxyl radical and hydrogen peroxide are the reactive oxygen species (ROS) considered most responsible for producing oxidative stress in cells and organisms.

Singlet oxygen interacts preferentially with guanine to produce 8-oxo-7,8-dihydroguanine and spiroiminodihydantoin.

DNA damage due to the latter lesion has not been detected directly in the DNA of cells exposed to singlet oxygen.

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(Kanofsky, J.R.; Sima, P. (Department of Veterans Affairs Hospital, Hines, IL (USA):

The reaction of ozone with a number of biological molecules was found to produce singlet oxygen in high yield. At pH 7.0, the reaction of ozone with an equimolar amount of biological molecule produced the following singlet oxygen yields (mole of singlet oxygen/mole of ozone): cysteine: 0.49 +/- 0.02; methionine: 1.13 +/- 0.11; reduced glutathione: 0.33 +/- 0.02; albumin: 1.00 +/- 0.05; uric acid: 0.64 +/- 0.09; ascorbic acid: 0.96 +/- 0.007; NADPH: 1.07 +/- 0.07; NADH: 0.95 +/- 0.01. )

( Full Article: 1O2 Singlet Oxygen Production from the Reactions of O3 Ozone with Biological Molecules )

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In this study, the singlet oxygen-induced lesion was isolated from a short synthetic oligomer after exposure to UVA radiation in the presence of methylene blue. The lesion could be enzymatically excised from the oligomer in the form of a modified dinucleoside monophosphate.

Using liquid chromatography-tandem mass spectrometry (LC-MS/MS), the singlet oxygen lesion was detected in the form of modified dinucleoside monophosphates in double-stranded DNA and in the DNA of HeLa cells exposed to singlet oxygen.

Pentamer containing the singlet oxygen-induced lesion and an isotopic label was synthesized as an internal standard for quantifying the lesion and served as well as for correcting for losses of product during sample preparation.

Received: September 1, 2005; Accepted: December 16, 2005

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